Pathways

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BRENDA KEGG MetaCyc

macrolide antibiotic biosynthesis (BRENDA)

:= BRENDA, := KEGG, := MetaCyc, := SABIO-RK
:= amino acid sequences := show the reaction diagram
EC Number
Reaction
Pathways
Reaction IDs
Stoichiometry Check
Missing Substrate
Missing Product
Commentary
Remark
pikromycin synthase
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = pikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
generic compounds
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: CYP107L1
natural substrates, generic, protein
pikromycin synthase
narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = neopikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
generic compounds
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-
natural substrates, generic, protein
narbonolide synthase
malonyl-CoA + 6 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ = narbonolide + 7 CoA + 7 CO2 + 5 NADP+ + 2 H2O
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: type I polyketide synthase, PikA1-4
natural substrates
10-deoxymethynolide desosaminyltransferase
TDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + narbonolide = dTDP + narbomycin
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: glycosyl transferase,DesVII
natural substrates
dTDP-4-amino-4,6-dideoxy-D-glucose transaminase
dTDP-4-amino-4,6-dideoxy-alpha-D-glucose + 2-oxoglutarate = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + L-glutamate
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natural substrates
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose transaminase
dTDP-4-amino-4,6-dideoxy-alpha-D-glucose + 2-oxoglutarate = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + L-glutamate
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natural substrates
dTDP-4-amino-4,6-dideoxy-D-glucose ammonia-lyase
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine + reduced acceptor = dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine + acceptor
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: radical SAM
: The enzyme is involved in biosynthesis of TDP-D-desosamine. It contains an [4Fe-4S] cluster [19746907]. This enzyme is a member of the 'AdoMet radical' (radical SAM) family. The reaction starts by using an electron from the reduced form of the enzyme's [4Fe-4S] cluster to split S-adenosyl-L-methionine into methionine and the radical 5-deoxyadenosin-5'-yl.
natural substrates, generic
dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose transaminase
dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose + 2-oxoglutarate = dTDP-3-dehydro-4,6-deoxy-alpha-D-glucose + L-glutamate
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natural substrates
dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucopyranose N,N-dimethyltransferase
2 S-adenosyl-L-methionine + dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucopyranose = 2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-3,4,6-trideoxy-alpha-D-glucopyranose
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natural substrates
dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase
2 S-adenosyl-L-methionine + dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucopyranose = 2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-3,4,6-trideoxy-alpha-D-glucopyranose
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natural substrates
3-alpha-mycarosylerythronolide B desosaminyl transferase
dTDP-D-desosamine + 3-alpha-L-mycarosylerythronolide B = dTDP + erythromycin D
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: desosaminyltransferase, EryCIII+EryCII, MegCIII+MegCII
natural substrates
erythromycin 12-hydroxylase
erythromycin D + NADPH + H+ + O2 = erythromycin C + NADP+ + H2O
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: 1200-1900-fold preference for erythromycin D over the alternative substrate erythromycin B
natural substrates
erythromycin 3''-O-methyltransferase
S-adenosyl-L-methionine + erythromycin C = S-adenosyl-L-homocysteine + erythromycin A
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: EryG: O-methyltransferase
natural substrates
erythromycin 3''-O-methyltransferase
S-adenosyl-L-methionine + erythromycin D = S-adenosyl-L-homocysteine + erythromycin B
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: EryG: O-methyl transferase
natural substrates
erythromycin 12-hydroxylase
erythromycin B + NADPH + H+ + O2 = erythromycin A + NADP+ + H2O
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: 1200-1900-fold preference for erythromycin D over the alternative substrate erythromycin B
: The enzyme hydroxylates the C12 position of the macrolactone ring of erythromycin, converting CPD-13806 and CPD-13805 to CPD-13951 and CPD-13804, respectively [8416893].
natural substrates
erythronolide mycarosyltransferase
dTDP-beta-L-mycarose + erythronolide B = dTDP + 3-alpha-L-mycarosylerythronolide B
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natural substrates
6-deoxyerythronolide B hydroxylase
6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
generic compounds
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natural substrates, generic, protein
6-deoxyerythronolide-B synthase
propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+ = 6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+
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: EryA MegA multi-step reaction
natural substrates, multi-step reaction
10-deoxymethynolide synthase
malonyl-CoA + 5 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ = 10-deoxymethynolide + 6 CoA + 6 CO2 + 5 NADP+ + 2 H2O
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: type I polyketide synthase,PikA1-3
natural substrates
10-deoxymethynolide desosaminyltransferase
dTDP-3-dimethylamino-3,4,6-trideoxy-alpha-D-glucopyranose + 10-deoxymethynolide = dTDP + 10-deoxymethymycin
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: glycosyl transferase,DesVII
natural substrates
pikromycin synthase
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = neomethymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
generic compounds
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: CYP107L1
natural substrates, generic, protein
pikromycin synthase
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
generic compounds
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: CYP107L1
natural substrates, generic, protein
pikromycin synthase
10-deoxymethymycin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + 2 O2 = novamethymycin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
n.a.
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natural substrates, protein